Mutational Analysis of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase
نویسندگان
چکیده
منابع مشابه
Mutational analysis of bovine viral diarrhea virus RNA-dependent RNA polymerase.
Recombinant bovine viral diarrhea virus (BVDV) nonstructural protein 5B (NS5B) produced in insect cells has been shown to possess an RNA-dependent RNA polymerase (RdRp) activity. Our initial attempt to produce the full-length BVDV NS5B with a C-terminal hexahistidine tag in Escherichia coli failed due to the expression of insoluble products. Prompted by a recent report that removal of the C-ter...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1999
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.73.12.10129-10136.1999